[seminar] Posdsjetnik na današnji seminar na IF-u petak, 18. 10. 2019. u 15:00h - Dr. Szymon Malinowski

[Damir Dominko]

****

*Institut za fiziku, Bijenička cesta 46,*

*predavaonica u zgradi Mladen Paić*

**petak, 18. 10. 2019. u 15:00 sati*
*
**
*Soft Plasma Polymerization technique in laccase-based biosensor 
construction*
*Dr. Szymon Malinowski*
*Lublin University of Technology*
The seminar will be focused on application new, plasma technique for 
deposition of enzyme bio-recognition layers and in consequence biosensor 
construction. This technique called Soft Plasma Polymerization (SPP) is 
based on low energy density Corona Discharge of plasma generated between 
two spark electrodes in presence of Helium carrier gas. Plasma 
deposition process of enzyme bio-recognition layer is consisted of two 
steps: (I) polymerization/cross-linking biological precursor inside 
plasma zone and (II) binding of polymerized/cross-linked biologically 
active molecules. So far I carried out optimization of plasma parameters 
(voltage, helium carrier gas flow rate) and process parameters 
(deposition time, biological precursor flow rate) optimization for 3 
solid supports i.e. glass slides, graphene oxide and multi-walled carbon 
nanotubes. Then, I applied SPP technique in construction of 
laccase-based electrochemical biosensors. Constructed biosensors were 
applied in determination pharmaceutics i.e. rutin and dopamine and 
environmental pollutions i.e. catechol, hydroquinone and resorcinol. The 
electrochemical measurement showed that application of SPP technique 
allows on significant reduction time needed to construction without 
losing analytical parameters of devices. Therefore, the SPP technique 
has high commercial value because can trade up to currently ways of 
biosensors construction called "wet" methods. As literature indicates 
"wet" methods are complex, time-consuming and in many cases needs 
long-hour conditioning of transducer element in enzyme solution in order 
to deposit of bio-recognition layer.

******Voditelji seminara IF-a: Ivan Balog <mailto:balog at ifs.hr> i Damir 
Dominko <mailto:ddominko at ifs.hr>**

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