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<div style="color: rgb(0, 0, 0); font-family: "times new
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style="font-size:16pt;color:black" lang="HR"><img
src="cid:part1.4649C1E2.E7585466@ifs.hr"
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<p style="text-align:center;margin:0px" align="center"><b><span
style="font-family:'century gothic' ,
sans-serif;color:#5f5f5f" lang="EN-GB">Institut za
fiziku, Bijenička cesta 46,</span></b></p>
<p style="text-align:center;margin:0px" align="center"><b><span
style="font-family:'century gothic' ,
sans-serif;color:#5f5f5f" lang="EN-GB">predavaonica u
zgradi Mladen Paić</span></b></p>
<strong><b><span style="font-size:14pt;font-family:'century
gothic' , sans-serif;color:#993300" lang="EN-GB">petak,
18. 10. 2019. u 15:00 sati</span></b><br>
</strong></div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"><strong></strong> </div>
<div style="text-align: center;"><span style="color: rgb(85,
145, 60); font-family: "times new roman", serif;
font-size: 12pt;"><span style="font-size:21.3333px"><b><font
face="times new roman, serif">Soft Plasma
Polymerization technique in laccase-based biosensor
construction</font></b></span></span><font face="times
new roman, serif" color="#55913c"><span style="font-size:
21.3333px;"></span></font></div>
<div style="text-align: center;"><font face="century gothic,
sans-serif"><span style="font-size: 18.6667px;"><b>Dr.
Szymon Malinowski</b></span></font></div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"> </div>
<div style="text-align: center;">
<div style="text-align: center;"><font face="century gothic,
sans-serif"><span style="font-size: 14.6667px;"><b>Lublin
University of Technology</b></span></font><span
style="font-size:small;font-family:'arial'"></span>
</div>
</div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"><span
style="font-size:small;font-family:'arial'"></span> </div>
The seminar will be focused on application new, plasma technique
for deposition of enzyme bio-recognition layers and in
consequence biosensor construction. This technique called Soft
Plasma Polymerization (SPP) is based on low energy density
Corona Discharge of plasma generated between two spark
electrodes in presence of Helium carrier gas. Plasma deposition
process of enzyme bio-recognition layer is consisted of two
steps: (I) polymerization/cross-linking biological precursor
inside plasma zone and (II) binding of polymerized/cross-linked
biologically active molecules. So far I carried out optimization
of plasma parameters (voltage, helium carrier gas flow rate) and
process parameters (deposition time, biological precursor flow
rate) optimization for 3 solid supports i.e. glass slides,
graphene oxide and multi-walled carbon nanotubes. Then, I
applied SPP technique in construction of laccase-based
electrochemical biosensors. Constructed biosensors were applied
in determination pharmaceutics i.e. rutin and dopamine and
environmental pollutions i.e. catechol, hydroquinone and
resorcinol. The electrochemical measurement showed that
application of SPP technique allows on significant reduction
time needed to construction without losing analytical parameters
of devices. Therefore, the SPP technique has high commercial
value because can trade up to currently ways of biosensors
construction called "wet" methods. As literature indicates "wet"
methods are complex, time-consuming and in many cases needs
long-hour conditioning of transducer element in enzyme solution
in order to deposit of bio-recognition layer. <br>
<br>
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<div style="color: rgb(0, 0, 0); font-family: arial, helvetica,
sans-serif; font-size: 12pt; text-align: left;"><b><span
style="font-size:12pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB"><b><span
style="font-size:11pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB"> </span></b></span></b><b><span
style="font-size:12pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB"><b><span
style="font-size:11pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB">Voditelji seminara IF-a: <a
href="mailto:balog@ifs.hr">Ivan Balog</a> i <a
href="mailto:ddominko@ifs.hr">Damir Dominko</a></span></b></span></b></div>
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