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<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"><strong><b><span
style="font-size:16pt;color:black" lang="HR"><img
src="cid:part1.E4278345.A60B144D@ifs.hr"
data-mce-src="imap://ddominko@mail.ifs.hr:143/fetch%3EUID%3E/INBOX%3E40129?header=quotebody&part=1.1.2.2&filename=logo_IF_seminar.jpg"
class=""></span></b></strong></div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;">
<p style="text-align:center;margin:0px" align="center"><b><span
style="font-family:'century gothic' ,
sans-serif;color:#5f5f5f" lang="EN-GB">Institut za fiziku,
Bijenička cesta 46,</span></b></p>
<p style="text-align:center;margin:0px" align="center"><b><span
style="font-family:'century gothic' ,
sans-serif;color:#5f5f5f" lang="EN-GB">predavaonica u
zgradi Mladen Paić</span></b></p>
<strong><b><span style="font-size:14pt;font-family:'century
gothic' , sans-serif;color:#993300" lang="EN-GB">petak,
18. 10. 2019. u 15:00 sati</span></b><br>
</strong></div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"><strong></strong> </div>
<div style="text-align: center;"><span style="color: rgb(85, 145,
60); font-family: "times new roman", serif;
font-size: 12pt;"><span style="font-size:21.3333px"><b><font
face="times new roman, serif">Soft Plasma Polymerization
technique in laccase-based biosensor construction</font></b></span></span><font
face="times new roman, serif" color="#55913c"><span
style="font-size: 21.3333px;"></span></font></div>
<div style="text-align: center;"><font face="century gothic,
sans-serif"><span style="font-size: 18.6667px;"><b>Dr. Szymon
Malinowski</b></span></font></div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"> </div>
<div style="text-align: center;">
<div style="text-align: center;"><font face="century gothic,
sans-serif"><span style="font-size: 14.6667px;"><b>Lublin
University of Technology</b></span></font><span
style="font-size:small;font-family:'arial'"></span> </div>
</div>
<div style="color: rgb(0, 0, 0); font-family: "times new
roman", "new york", times, serif; font-size:
12pt; text-align: center;"><span
style="font-size:small;font-family:'arial'"></span> </div>
The seminar will be focused on application new, plasma technique
for deposition of enzyme bio-recognition layers and in consequence
biosensor construction. This technique called Soft Plasma
Polymerization (SPP) is based on low energy density Corona
Discharge of plasma generated between two spark electrodes in
presence of Helium carrier gas. Plasma deposition process of
enzyme bio-recognition layer is consisted of two steps: (I)
polymerization/cross-linking biological precursor inside plasma
zone and (II) binding of polymerized/cross-linked biologically
active molecules. So far I carried out optimization of plasma
parameters (voltage, helium carrier gas flow rate) and process
parameters (deposition time, biological precursor flow rate)
optimization for 3 solid supports i.e. glass slides, graphene
oxide and multi-walled carbon nanotubes. Then, I applied SPP
technique in construction of laccase-based electrochemical
biosensors. Constructed biosensors were applied in determination
pharmaceutics i.e. rutin and dopamine and environmental pollutions
i.e. catechol, hydroquinone and resorcinol. The electrochemical
measurement showed that application of SPP technique allows on
significant reduction time needed to construction without losing
analytical parameters of devices. Therefore, the SPP technique has
high commercial value because can trade up to currently ways of
biosensors construction called "wet" methods. As literature
indicates "wet" methods are complex, time-consuming and in many
cases needs long-hour conditioning of transducer element in enzyme
solution in order to deposit of bio-recognition layer. <br>
<br>
</div>
<div style="color: rgb(0, 0, 0); font-family: arial, helvetica,
sans-serif; font-size: 12pt; text-align: left;"><b><span
style="font-size:12pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB"><b><span
style="font-size:11pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB"> </span></b></span></b><b><span
style="font-size:12pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB"><b><span
style="font-size:11pt;font-family:'century gothic' ,
sans-serif" lang="EN-GB">Voditelji seminara IF-a: <a
href="mailto:balog@ifs.hr">Ivan Balog</a> i <a
href="mailto:ddominko@ifs.hr">Damir Dominko</a></span></b></span></b></div>
<pre class="moz-signature" cols="72">--
Damir Dominko
Research associate
Institute of Physics Zagreb
Bijenicka cesta 46, 02-226
10000 Zagreb, Croatia
office: +385 1 469 8821
cell: +385958172990
e-mail: <a class="moz-txt-link-abbreviated" href="mailto:ddominko@ifs.hr">ddominko@ifs.hr</a></pre>
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