[seminar] Seminar na IF-u u ponedjeljak 20.11.2017. u 15:00h - dr. Aleksandar Krmpot (Beograd)

[Damir Staresinic]

Institut za fiziku, Bijenička cesta 46, 

predavaonica u zgradi Mladen Paić ponedjeljak, 20. studenog 2017., u 15:00 sati 
Microworld in 3D - imaging by nonlinear scanning microscopy 
dr. Aleksandar J. Krmpot 
Centar za fotoniku, Institut za fiziku, Beograd-Zemun, Srbija 



The field of optical microscopic imaging is rapidly developing and it is the playground for interception between physics, chemistry and biology. The importance of advanced microscopic techniques is supported by The Nobel prize for Chemistry for 2014 that is awarded for the development of super-resolved fluorescence microscopy. Nonlinear laser scanning microscopy (NLSM) is optical technique that utilize ultrashort laser pulses for 3D imaging of microscopic samples. Three modalities of NLSM, two photon excitation fluorescence (TPEF), second and third harmonic generation (SHG/THG), are often used for label free and in vivo imaging. NLSM has possibility for deep penetration into the sample volume with reduced side effects like photo bleaching and photo toxicity. The basic physical principles of the three NLSM modalities (TPEF, SHG and THG) will be presented in the talk and also their application for 3D imaging at tissue and single cell level. 



1. B. R. Masters, and P. So, Handbook of Biomedical Nonlinear Optical Microscopy, Oxford University Press (2008) and references therein. 

2. Katarina Bukara, Svetlana Jovanić, Ivana T. Drvenica, Ana Stančić, Vesna Ilić, Mihailo D. Rabasović, Dejan Pantelić, Branislav Jelenković, Branko Bugarski, Aleksandar J. Krmpot 
MAPPING OF HEMOGLOBIN IN ERYTHROCYTES AND ERYTHROCYTE GHOSTS USING TWO PHOTON EXCITATION FLUORESCENCE MICROSCOPY 
JOURNAL OF BIOMEDICAL OPTICS 22(2), 026003 (2017). 

3. Sanja Z. Despotović, Novica M. Milićević, Dragoslav P. Milošević, Nebojša Despotović, Predrag Erceg, Petar Svorcan, Udo Schumacher, Sebastian Ullrich, Gordana Mihajlović, Dragan Kalem, Srđan Marković, Ivana M. Lalić, Aleksandar J. Krmpot , Mihailo D. Rabasovic, Dejan V. Pantelić, Svetlana Z. Jovanić, Thomas Rösch, Živana Milićević 
REMODELING OF EXTRACELLULAR MATRIX OF THE LAMINA PROPRIA IN THE UNINVOLVED HUMAN RECTAL MUCOSA 10 CM AND 20 CM AWAY FROM THE MALIGNANT TUMOR 
TUMOR BIOLOGY 39 (7), 1010428317711654 (2017). 

4. Pantelic Dejan, V Savic-Sevic Svetlana, N Stojanovic Dejan, V Curcic, Srecko B, Krmopot Aleksandar , J Rabasovic Mihailo, D Pavlovic Danica, Lazovic Vladimir Milosevic Vojislav 
SCATTERING-ENHANCED ABSORPTION AND INTERFERENCE PRODUCE A GOLDEN WING COLOR OF THE BURNISHED BRASS MOTH, DIACHRYSIA CHRYSITIS 
PHYSICAL REVIEW E 95 032405 (2017). 

5. Mihailo D. Rabasović, Dejan V. Pantelić, Branislav M. Jelenković, Srećko B. Ćurčić, Maja S. Rabasović, Maja D. Vrbica, Vladimir M. Lazović, Božidar P. M. Ćurčić, Aleksandar J. Krmpot 
NONLINEAR MICROSCOPY OF CHITIN AND CHITINOUS STRUCTURES: A CASE STUDY OF TWO CAVE-DWELLING INSECTS 
JOURNAL OF BIOMEDICAL OPTICS_20 016010 (2015). 

6. Aleksandar J. Krmpot , George J Tserevelakis, Branka D Murić, George, Filippidis, and Dejan V Pantelić 
3D IMAGING AND CHARACTERIZATION OF MICROLENSES AND MICROLENSES ARRAYS USING NONLINEAR MICROSCOPY 
JOURNAL OF PHYSICS D 46, 195101 (2013). 

7. J. Tserevelakis, G. Filippidis, A.J. Krmpot , M. Vlachos, C. Fotakis, N. Tavernarakis 
IMAGING CAENORHABDITIS ELEGANS EMBRYOGENESIS BY THIRD-HARMONIC GENERATION MICROSCOPY 
MICRON, 41 444 (2010). 




Voditelji seminara IF-a: [ mailto:hrvoje at ifs.hr | Hrvoje Skenderović ] i [ mailto:damirs at ifs.hr | Damir Starešinić ] 

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